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Schematic illustration of the workflow for Hyalomma dromedarii transcriptome mining starting from tick colony preparation, collecting the developmental stages, RNA isolation followed by complementary DNA ( cDNA ) library construction and finally sequencing and analysis of transcripts

Journal: Parasites & Vectors

Article Title: Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens

doi: 10.1186/s13071-024-06538-5

Figure Lengend Snippet: Schematic illustration of the workflow for Hyalomma dromedarii transcriptome mining starting from tick colony preparation, collecting the developmental stages, RNA isolation followed by complementary DNA ( cDNA ) library construction and finally sequencing and analysis of transcripts

Article Snippet: The pCMV-SPORT6.1 vector (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) was used to ligate complementary DNA (cDNA) fragments ≥ 800 bp for construction of the H. dromedarii cDNA library in pCMV-SPORT6.1 according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific) and applying the basic protocols reported in Sambrook and Russell [ ].

Techniques: Isolation, cDNA Library Assay, Sequencing

Hyalomma dromedarii complementary DNA (cDNA) after Cap-antibody selection. A Lanes: 1 1 kb+ DNA ladder, 2 Cap-antibody-selected cDNA from tick. B Lanes: 1 1 kb+ DNA ladder, 2 Cap-antibody-selected cDNA from human kidney as a control

Journal: Parasites & Vectors

Article Title: Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens

doi: 10.1186/s13071-024-06538-5

Figure Lengend Snippet: Hyalomma dromedarii complementary DNA (cDNA) after Cap-antibody selection. A Lanes: 1 1 kb+ DNA ladder, 2 Cap-antibody-selected cDNA from tick. B Lanes: 1 1 kb+ DNA ladder, 2 Cap-antibody-selected cDNA from human kidney as a control

Article Snippet: The pCMV-SPORT6.1 vector (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) was used to ligate complementary DNA (cDNA) fragments ≥ 800 bp for construction of the H. dromedarii cDNA library in pCMV-SPORT6.1 according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific) and applying the basic protocols reported in Sambrook and Russell [ ].

Techniques: Selection, Control

Abundance and length of SMaT-encoding sequences in the Hyalomma dromedarii complementary DNA (cDNA) library. A Sequence length distribution of the 319 SMaT-encoding sequences used in the analysis. B Percentage of H. dromedarii SMaT-encoding sequences that had matched hits with other ticks according to alignment using blastx. SMaT, Secreted, membrane-associated or transmembrane

Journal: Parasites & Vectors

Article Title: Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens

doi: 10.1186/s13071-024-06538-5

Figure Lengend Snippet: Abundance and length of SMaT-encoding sequences in the Hyalomma dromedarii complementary DNA (cDNA) library. A Sequence length distribution of the 319 SMaT-encoding sequences used in the analysis. B Percentage of H. dromedarii SMaT-encoding sequences that had matched hits with other ticks according to alignment using blastx. SMaT, Secreted, membrane-associated or transmembrane

Article Snippet: The pCMV-SPORT6.1 vector (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) was used to ligate complementary DNA (cDNA) fragments ≥ 800 bp for construction of the H. dromedarii cDNA library in pCMV-SPORT6.1 according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific) and applying the basic protocols reported in Sambrook and Russell [ ].

Techniques: cDNA Library Assay, Sequencing, Membrane